High glucose-induced bovine retinal microvascular pericytes FOXO1A increased expression of.docVIP

 PAGE \* MERGEFORMAT 11 High glucose-induced bovine retinal microvascular pericytes FOXO1A increased expression of 【Abstract】 Objective: To explore the high glucose in cultured bovine retinal capillary pericytes FOXO1A expression. Methods: Primary cultured pericytes using immunocytochemistry and Western blot used to detect high glucose-induced bovine retinal microvascular pericytes FOXO1A protein. Results: The retinal capillary pericytes in the 5mmol / L D-glucose after 72h culture, FOXO1A immunoreactivity mainly in the nucleus, in 30mmol / L D-glucose or 5μ mol / L H2O2, after 72h culture, FOXO1A a significant increase in immunoreactivity , FOXO1A immunoreactivity mainly in the nucleus and cytoplasm; Western blot found that high glucose group and the hydrogen peroxide group of cell culture 48,72 h weeks after FOXO1A protein activity and expression than the control group increased significantly. Conclusion: High glucose-induced retinal capillary pericytes FOXO1A expression and activity increased significantly. Keywords: Glucose FOXO1A protein expression in pericytes Up-expression of FOXO1A in bovine retinal microvascular pericytes induced by glucose Abstract AIM: To study the expression of FOXO1A in bovine retinal microvascular pericytes cultured with the high glucose medium. METHODS: Primary bovine retinal microvascular pericytes were cultured. FOXO1A expression was measured in bovine retinal microvascular pericytes treated with low glucose (5 mmol / L) , high glucose (30mmol / L) and H2O2 (5μmol / L) in medium containing 20mL / L fetal bovine serum using immunocytochemistry and Western Blot analysis.RESULTS: Slight FOXO1A immunoreactivity was detected the retinal microvascular pericytes treated with low glucose after 72 hours distributed in the nucleus. FOXO1A immunoreactivity was significantly increased in the retinal microvascular pericytes treated with high glucose and H2O2 after 72 hours and distributed in the nucleus and cytop