High Performance Liquid Chromatography in Mongolian Medicine yellow clematis content of quercetin.docVIP

 PAGE \* MERGEFORMAT 10 High Performance Liquid Chromatography in Mongolian Medicine yellow clematis content of quercetin Author: Chang Fu-hou Qijun Fan Lei Han Ruilan YIN Qin Min-Jie Wang [Abstract] Objective To determine the yellow Clematis in the quercetin content in the Mongolian medicine yellow clematis to provide the content of quercetin in detection methods, develop quality control standards. Methods using high performance liquid chromatography, Kromasil (250 mm * 4.6 mm, 5 μ m) column, methanol  0.4% phosphoric acid (60:40) as mobile phase, flow rate of 1.0 ml / min; detection wavelength was 360 nm. Results quercetin concentration 15.35 ~ 76.75 μ g / ml and the peak area between the scope of a good linear relationship, r = 0.999 6. Quercetin average recovery was 98.13%, RSD = 1.55%. Conclusions The high-performance liquid chromatography yellow clematis content of quercetin in a good separation, simple and accurate. [Keywords:] yellow clematis Endothelin high-performance liquid chromatography assay RANUNCULACEAE Clematis flower to flower Clematis Clematis intricata Bge.’s Aerial parts, mainly distributed in Inner Mongolia, Liaoning, Hebei, Shanxi, Shaanxi, Gansu, Qinghai and other places, many born in the hillside, grassland, roadside or bush a pair. ‘Yellow Clematis’ names from their plant names, as a Mongolian medicine’ alag - Te Mu Er - Ao Yang ancient Japan ‘income is contained in the’ China Medical Encyclopedia ‘(Mongolian sub-volumes)’ to identify drug BAI Jing Kam Kam’’correct’’Mongolian Medicine, Inner Mongolia Chinese herbal medicine ‘and’ Inner Mongolia herbal chi ‘[1]’ Chinese Materia Medica ‘(Mongolian medicine volume). Attending the stomach swelling of the liver, stone swelling of the liver, colon swelling of the liver, swelling of the liver and other cold water swelling of the liver disease. Existing studies have shown that quercetin has anti-inflammatory, anti-oxidation, anti-cancer and anti-mutation, anti-cardiac hypertrophy and b