HPLC Analysis of Hypericum perforatum in different parts of the content of Hypericum perforatum.docVIP

 PAGE \* MERGEFORMAT 6 HPLC Analysis of Hypericum perforatum in different parts of the content of Hypericum perforatum Abstract The HPLC determination of Hypericum perforatum roots, stems, leaves, flowers, fruit in hypericin content; The results showed that the highest content of flowers, stems little in content. Reposted elsewhere in the paper for free download Origin Flower Ye Stem Root Fruit Herba Wen County, Gansu 0.072 0.046 0.002 Undetected 0.028 Kang County in Gansu 0.090 0.035 Undetected Undetected 0.039 Hunan Longshan 0.058 0.037 0.007 Undetected 0.019 0.019 Guizhou Bijie 0.057 0.036 0.003 Undetected 0.009 0.022 Shaanxi Danfeng 0.086 0.033 Undetected Undetected 0.018 Enshi, Hubei Province 0.085 0.040 Undetected Undetected 0.015 4Chuan Dachuan 0.050 0.035 Undetected Undetected 0.018 2.6 plus precision sample recovery trial to take medicine that powder 3 copies, respectively, by adding different amounts of hypericin reference substance, according to the sample determination operation, hypericin average recovery 100.3%, RSD = 4.17%. 3 Discussion Determination of hypericin ① available 〔 2〕 TLC or HPLC method with fluorescence detection and gradient elution 〔 3〕 . The established HPLC method using UV detector, and for the other degree-eluting compared with the above-mentioned method is simple, practical and has a strong operational. ② from the assay results suggest: hypericin in the flower of the highest concentrations, followed by leaf, fruit content is low, very little content in stem and root were not detected in hypericin. And should therefore be harvested in the flowering aerial parts, abandoned to the stem; medicinal quality to mosaic more superior to those. References 〔 1〕 Ezio Bombardelli, Paolo Morazzoni. Hypericum perforatum. Fitoterapia, 1995 (1): 43 〔 2〕 Kartnig Th and Gbel I. Determination of hypericin and pseudohypericin by thin laye