HPLC determination of adenosine content in the fruiting bodies of Cordyceps militaris.docVIP

 PAGE \* MERGEFORMAT 10 HPLC determination of adenosine content in the fruiting bodies of Cordyceps militaris [Abstract] Objective To determine the content of adenosine in the fruiting bodies of Cordyceps militaris method. Methods using high performance liquid chromatography column was 18 alkyl silane bonded silica gel column (4.6 mm * 250 mm, 5μ m); mobile phase: water  methanol (volume ratio 85:15), flow rate: 1.2 mL / min ; detection wavelength: 260 nm. Results The average recovery of adenosine 100.23%, RSD = 1.43%. Conclusion The method is simple, accurate and applicable to the analysis of product quality inspection. [Keywords:] high-performance liquid chromatography; Cordyceps fruiting body; Adenosine Abstract: Objective To establish an HPLC method for content determination of adenosin in Cordyceps militaris (L.) Link. Methods Samples were analyzed on a DiamonslTM C18 column (4.6 mm * 250 mm, 5 μ m) by using water  methanol (85:15 ) as mobile phase. The detection wavelength was 260 nm. Results The average recovery of adenosine was 100.23% with RSD = 1.43%. Conclusion The method is simple, accurate, which can be used for the quality control of Cordyceps militaris (L.) Link. Keywords:: HPLC; Cordyceps militaris (L.) Link; adenosine Cordyceps militaris (also known as the north Chinese caterpillar fungus, Cordyceps) for the ergot fungus fruiting branches pupa grass fungus Cordyceps Cordyceps. Militaris (L.) Link.’s Dry sub-block is the type species of the genus Cordyceps is also very similar with the Cordyceps sinensis species. Research data indicate that the active ingredient in Cordyceps militaris fruiting bodies similar to wild Cordyceps sinensis, contains large amounts of cordycepin, adenosine, adenine, Cordyceps acid, such as Chinese caterpillar fungus polysaccharide [1-3]. Are reported in the literature, adenosine can reduce the leakage of myocardial enzymes, to maintain the integrity of the cell membrane and reduce coronary resistance and my