Injection pain must ligation injury in rats with chronic spinal cord dorsal horn c-fos and substance P effects.docVIP

 PAGE \* MERGEFORMAT 10 Injection pain must ligation injury in rats with chronic spinal cord dorsal horn c-fos and substance P effects 【Abstract】 Objective pain must be injection is a good analgesic effect of Chinese medicine, to be the passage of this experimental study of its analgesic mechanism. Methods Immunohistochemical methods to study the pain must be injection CCI (chronic ligation injury) model of rat spinal cord dorsal horn c-fos gene expression and P substance contents in. Results pain injection will significantly reduce the CCI rat spinal cord dorsal horn c-fos gene and P substances. Conclusion This experiment confirmed that the pain injection on the CCI will have a very good analgesic effect in rats, reducing the spinal cord dorsal horn neurons to incoming nociceptive stimuli, may be the analgesic effect of one of the ways to play. Keywords: must be injection pain in c-fos P substance Immunohistochemistry Injection pain must be from the four kinds of traditional Chinese medicine (active ingredient or parts) consisting of a proprietary Chinese medicine has a good analgesic effect. The animal experiments and clinical application of both display and meperidine [1,2] has a similar analgesic effect, but no significant addictive. Produced by mechanism of its analgesic effect has not yet entirely clear, and recent studies have shown that peripheral noxious stimulation in spinal cord and other factors can activate immediate early gene c-fos and expression of fos protein [3 ~ 6]; P substance (substance P, SP ) are considered to be spinal nociceptive transmission of material [7], in the spinal cord pain transmission of information played an important role in [8], both at the molecular level is a sign of pain. In this study, using immunohistochemical staining method of pain must be injection CCI (chronic constriction injur, chronic ligation injury) model of rat spinal cord superficial dorsal horn c-fos gene expression and SP content i