Inonotus obliquus extract on human gastric cancer cell lines BGC823 induction of apoptosis and anti-proliferation mechanism.docVIP

 PAGE \* MERGEFORMAT 11 Inonotus obliquus extract on human gastric cancer cell lines BGC823 induction of apoptosis and anti-proliferation mechanism Of: Zhong Xiuhong Sun Dong-sik Choi Ji-Cheng Gao Yu YANG Shu-yan [Abstract] Objective To study extracts of Inonotus obliquus in vitro on human gastric cancer cell lines BGC 823 induced apoptosis and anti-proliferation mechanism. Methods TUNEL method and immunohistochemistry staining Inonotus obliquus extract on gastric cancer cell line BGC 823. Results 10 ~ 80 mg / L extract of Inonotus obliquus role in human gastric cancer BGC 823 cells, the tumor cell apoptosis is associated with drug concentration, Ki 67 positive rate with the drug concentration reduce the increase, showing a concentration dependent manner .80 mg / L extract of Inonotus obliquus effect after 48 h, bcl 2 gene protein expression was significantly reduced, and bax, caspase 3 protein expression was significantly increased . Conclusions from Inonotus obliquus extract on human gastric carcinoma BGC 823 cell lines induced apoptosis and anti-proliferative effect, the molecular mechanisms of apoptosis may be regulated by down bcl 2 and bax expression, caspase apoptotic signal start achieved. [Keywords:] Inonotus obliquus, stomach cancer, apoptosis Obliquus is a major growth in the birch on the medicinal fungi, is a Basidiomycetes Polyporaceae Branch (1). In recent years, Inonotus obliquus chemical composition, pharmacological effects and mechanisms of the new aspects reports (2,3), in particular anti-tumor effect caused Russia, Scandinavia, Japan, South Korea and other countries, the United States and the relevant academic attention, in order to investigate the antitumor mechanism, the laboratory findings in the previous obliquus BGC 823 extract on gastric cancer cell proliferation inhibition and significant morphological changes of apoptosis of tumor cells (4). In order to further investigate the extract of Inonotus obliquus on human gastric