Ischemia reperfusion injury changes in nerve function and protein expression in the brain Homer1.docVIP

 PAGE \* MERGEFORMAT 16 Ischemia reperfusion injury changes in nerve function and protein expression in the brain Homer1 Of: Jiang Min, Ma Zhengliang, Gu Xiaoping, Juan Rivers [Abstract] Objective: To observe the focal ischemia-reperfusion (I / R) changes in neural function and brain Homer1a, Homer1b / c protein expression. Methods: 120 male SD rats were randomly divided into group (N group), sham operation group (S group) and I / R group, n = 40, I / R group produced by middle cerebral artery occlusion model; S group, only surgical operation, no wire embolism; N group normal feeding as blank control. respectively 6,12,24,48 and 72 h after injury using neural injury severity score (NSS) method on the 3 groups to evaluate nerve function, taking 0,1 h after reperfusion and the above the amount of time rat cortex and hippocampus homogenate cracking line Western blotting, on Homer1a and Homer1b / c Western blot analysis of protein line. Results: NSS shows I / R rats 6 h after reperfusion nerve injury in the most obvious (with the same group of other time points, P lt;0.001), and then gradually recovered, 72 h was close to the preoperative level (with N, S group, P lt;0.05). Western blotting results show that, I / R group of neural tissue Homer1a proteins from 0 h after injury after injury from continued until 72 h, while the normal and the sham group no positive expression of the protein; Homer1b / c can be seen in the 3 groups were continued expression. Conclusion: The rat I / R injury Homer1a protein expressed in a significant injury before and after the change Homer1b / c no, with NSS suggest Homer1a protein may be involved in cerebral I / R induced neurological injury. [Keywords:] ischemia-reperfusion; Homer protein; nerve pathology score; rat [Abstract] Objective: To observe the neurological changes and expression of protein Homer1a and Homer1b / c in rat brain with ischemia reperfusion insult. Methods: 120 male SD rats were randomly divided into 3