Ischemic postconditioning on ischemiareperfusion injury in rat intestinal mucosa role of the anti.docVIP

 PAGE \* MERGEFORMAT 6 Ischemic postconditioning on ischemiareperfusion injury in rat intestinal mucosa role of the anti Author: Chu Weiwei, Wu-step strong, sand-Hwan Chen, Dian-Hua Wang [Abstract] Objective To study the ischemic postconditioning on ischemia-reperfusion in rat intestinal mucosa  anti-injury. Methods to copy SD intestinal ischemia  reperfusion injury model. Rats were divided into 4 groups (n = 8): sham operation group (S), ischemia  reperfusion (I / R), ischemic preconditioning group (IPC), ischemia and post-treatment group (I  post). Comparison of intestinal mucosa in each group of malondialdehyde (MDA) content, superoxide dismutase (SOD) and myeloperoxidase (MPO) activity changes; terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling method (TUNEL) detection of the incidence of intestinal mucosa apoptosis; Chiu  s rates were observed damage of intestinal mucosa. Results with I / R group compared, IPC group and I  post rat intestinal mucosa MDA content and MPO activity were significantly reduced, while SOD activity was significantly increased; can be seen clearly after reperfusion apoptosis of intestinal mucosal cells, I  post group and the apoptotic index in IPC group I / R group was significantly decreased histopathological damage is significantly reduced. I  post group and IPC group compared with the indicators no significant difference. Conclusions Post-treatment ischemia-reperfusion by inhibiting excessive production of oxygen free radicals, protecting antioxidant system, thus inhibiting apoptosis of intestinal mucosa, reducing intestinal ischemia  reperfusion injury. [Keywords:] ischemia  reperfusion; ischemic post-processing; Ischemic preconditioning; apoptosis; intestinal mucosa Intestinal ischemia-reperfusion injury and clinical and pathological process of many diseases closely related to apoptosis in the pathological process of their injury play an important role [1  2]. 1986, Murry and so o