Intrathecal injection of Improvement and validation.docVIP

 PAGE \* MERGEFORMAT 6 Intrathecal injection of Improvement and validation [Keywords:] injections, spinal, bupivacaine, disease models, animal Intrathecal administration of study at the spinal level is an important means of drug action, because direct intrathecal injection method is simple, less interference factors, was used by a growing number of scholars. The traditional method for Mestre intrathecal injection method, It is based on action flick when needle puncture mark of success [1], but the author during the course of animal experiments and found that there is a dispute the judge signs this end, the author of the intrathecal injection method improved (ie New Law cerebrospinal fluid or by injection to withdraw sword after a flick action is successful puncture marks, and verify its reliability. 1 Materials and methods 1.1 General Information SD 60 rats, female, 9 to 11 weeks, body weight (320 +-26.5g (290 ~ 340g, from the Shanghai Experimental Animal Center of Chinese Academy of Sciences, Animal Certificate of Conformity SCXK (Shanghai) 2007 005, random were divided into 2 groups according to New France and Mestre method puncture, intrathecal injection of 0.5% bupivacaine. bupivacaine (Chaohui Shanghai Fuxing Pharmaceutical Company, isoflurane (Abbott Italy, 25 L micro injector (Glass Instrument Factory Shanghai Xin Gao, needle cut to 2.5 cm, pinhole diameter grinding to lt;1 mm, washed and autoclaved distilled water reserve. 1.2 Method New Method rats at 1.5% isoflurane anesthesia [2], take V seat to L5-6 spinous process space as the puncture point, puncture point and rats maintained the highest point of the back of the vertical distancegt; 3 cm. experimenter left hand and hip exploration nodules, and its horizontal position is the rat L5-6 spinous process gap. left thumb and middle finger placed in rats with L5-6 spinous process on both sides of the gap and tighten the skin outward. to the index finger position, right hand perpendicular to