Ischemic postconditioning on ischemic reperfusion in rats with high blood myocardial Bcl2 and Bax protein expression.docVIP

 PAGE \* MERGEFORMAT 13 Ischemic postconditioning on ischemic reperfusion in rats with high blood myocardial Bcl2 and Bax protein expression Of: Huo Yue Yin Boying LIU Sheng-hui Abstract Objective To observe the treatment of high blood lipids after ischemic myocardial ischemia reperfusion in rats Bcl 2 and Bax protein expression. Methods SD rats were 36 high cholesterol were randomly divided into 3 groups: sham operation group, ischemia-reperfusion group, ischemic postconditioning group, 12 in each group. Rat model of myocardial ischemia and reperfusion. R group: to tighten the ligature ischemia 40 min, relax ligature and reperfusion 240 min; ischemic postconditioning group: after 40 min ischemia, reperfusion 10 s, ischemia and 10 s, for 3 cycles, then reperfusion 240 min; sham operation group: thoracotomy threading do rings, but do not tighten the ligature. Since the end of reperfusion the right carotid artery blood serum creatine kinase (CK) activity was detected by TUNEL reperfusion degree of apoptosis, detected by immunohistochemistry Bcl 2 and Bax protein expression. Results serum CK activity assay: after the end of reperfusion after ischemia and reperfusion treatment group CK activity was significantly higher than sham operation group were (789.68 67.34), (932.86 84.17), (252.48 19.78) U / L, P lt;0.05, ischemic postconditioning group was significantly lower than IR group (P lt;0.05). count of apoptotic cells: the end of reperfusion the sham group no significant apoptosis (lt;5%), ischemic myocardial apoptosis after treatment was significantly lower than ischemia-reperfusion group were (11.9 2.7)%, (21.2 3.5)%, P lt;0.05. Compared with the ischemia-reperfusion, ischemic postconditioning group Bcl 2 expression increased (P lt;0.05), Bax protein expression reduced (P lt;0.05). Conclusion ischemic postconditioning can increase high blood myocardial ischemia and reperfusion Bcl 2 protein expression, reducing the expression of Bax pro