Iso-13-based diamine in vivo anti-tumor effect.docVIP

 PAGE \* MERGEFORMAT 7 Iso-13-based diamine in vivo anti-tumor effect Author: Wang Ruixing, Wong, rubidium, Wu Zhi-Juan, Wu territory, Lin Jing [Keywords:] antineoplastic agents;, tumor cells, cultured;, tumor transplantation;, iso-13-based diamine Abstract: Objective To observe the different 13-based diamine (D79) and in vivo anti-tumor effect. Methods trypan blue exclusion method, MTT assay D79 on a variety of tumor cells in vitro cytotoxicity; Bliss acute toxicity was observed by D79; in D79 can be tolerated dose observed in mice transplanted solid tumors of U14, ascites tumor EAC, HAC of the inhibition rate. Results D79 remarkable variety of in vitro anti-tumor cells, drug effects 48 h, IC50 1.24 ~ 3.36 μ g / mL; D79 of the LD50 of 36.49 mg / kg (intravenous injection). D79-suppressed mice implanted U14 in vivo solid tumor growth, enhance transplanted ascites tumor EAC, HAC role in the survival time of mice with a dose-dependent. Conclusion D79 have a stronger anti-tumor effect in vivo. Keywords:: antineoplastic agents; tumor cells, cultured; tumor transplantation; iso-13-based diamine ABSTRACT: Objective To observe the antitumor effect of allotritridecyldiamidogen (D79) in vivo and in vitro. Methods The cytotoxic effects of D79 on various tumor cells lines cultured in vitro were determined by trypan blue dye exclusion test, MTT method. Acute toxicity of D79 was evaluated by Bliss method: at a tolerable dose level, D79 was administrated to treat transplanted solid tumor U14 and ascetic tumors EAC and HAC. Tumor weight inhibition and life extension were observed. Results D79 induced significant growth arrest in various malignant tumor cell lines cultured in vitro. IC50 of these tumor cells exposed to the drug for 48 hours ranged from 1.24 ~ 3.36 μ g / mL. LD50 of D79 was 36.49 mg / kg (mice, iv). D79 inhibit in vivo growth of implanted solid tumor U14 and prolonged live time implanted ascetic tumors EAC and HAC of mice in a dosedependment manner. Conclu