Intrauterine hypoxia of different duration on fetal rat neural stem cell proliferation and the protective effect of Angelica sinensis.docVIP

 PAGE \* MERGEFORMAT 15 Intrauterine hypoxia of different duration on fetal rat neural stem cell proliferation and the protective effect of Angelica sinensis Author: Chen Yan Bing Cao Mei Xinming CAO Kun-Hong Yu [Abstract] Objective To study the process of intrauterine hypoxia on the embryonic rat neural stem cell proliferation and the protective effects of Angelica injection. Methods 15 d of SD pregnant rats were randomly divided into nine groups: control group, 3 h hypoxia group, hypoxia 6 h group, 9 h hypoxia group, hypoxia 12 h group, Angelica 3 h group, Angelica 6 h Group 9 h group and the angelica angelica 12 h groups of five. All Angelica pregnant rats via the tail vein injection of angelica injection, and then hypotonic hypoxia-induced fetal rat model of intrauterine hypoxia; all hypoxia group received saline instead of Angelica injection, the other with the angelica group; control group, only the injection salt water, not oxygen. Get fetal brain tissue for Nestin immunohistochemistry, image analysis. Results 3 h hypoxia group, hypoxia 6 h group and 9 h hypoxia group than in the control group staining positive cells increased, a greater number of positive cells in hypoxia 12 h group than in the control group reduced the hypoxia group comparison was statistically differences; In addition to Angelica 12 h group, the rest of the angelica group of positive stained cells in hypoxia group compared with the corresponding reduced the quantity reduced; angelica 12 h group and 12 h hypoxia was no significant difference between the two groups (Pamp;gt; 0.05). Conclusion When a certain process, a certain concentration of oxygen can stimulate the proliferation of neural stem cells into the state, the strength of their proliferation and hypoxia-term related to the proliferation of hypoxia and reached the peak 6 h, 9 h oxygen began to decrease; Long-term absence oxygen (12 h) will lead to reduction of neural stem cells; Angelica on a certain time-and oxygen-deficien