Isolation and Identification of water extract on human lung squamous cell carcinoma of the expression of Survivin.docVIP

 PAGE \* MERGEFORMAT 12 Isolation and Identification of water extract on human lung squamous cell carcinoma of the expression of Survivin Of: Xing Liqiang, Zhao Jianqing, Li Wang, Li Sun [Abstract] Objective To study the Isolation and Identification of water extract on human lung squamous cell carcinoma of NCI-H520 cells and inhibition of the expression of Survivin. Methods of Isolation and Identification with different concentrations of water extract on NCI-H520 cells MTT assay were used to inhibit the rate of lung cancer cells, semi-quantitative RT-PCR, the level of Survivin mRNA and protein by Western-blotting detection of expression level. Results Isolation and Identification of water extract can significantly inhibit the growth of NCI-H520 cells, with the drugs inhibitory concentration increased gradually. Isolation and Identification role of 4 h, cells reduce the level of Survivin protein expression, and the expression of Survivin mRNA did not change significantly. Conclusions Isolation and Identification of water extract inhibited the growth of NCI-H520 cells and a concentration and time dependent; Isolation and Identification of water extract can reduce the NCI-H520 cells, expression of Survivin protein, but no significant effect on the mRNA level. [Keywords:] Isolation and Identification of water extract; NCI-H520 cell line; Survivin Lung cancer is human life and health of the world against the most common malignant tumor, the incidence rate increasing year by year. In developed countries, lung cancer incidence and mortality ranking the first [1], lung cancer cases in the small Central Africa cell lung cancer (mainly squamous cell carcinoma, adenocarcinoma, large cell carcinoma) account for about 80%. Survivin has been reported in recent years in non-small cell lung cancer can cause resistance to chemotherapeutic drugs, thereby affecting the patient’s chemotherapy. In this study, water extract of Chinese medicine Isolation and Identificat