Kanamycin on isolated cochlear hair cell damage in rat experimental study.docVIP

 PAGE \* MERGEFORMAT 10 Kanamycin on isolated cochlear hair cell damage in rat experimental study Of: Li Ting Yu Wang Ping Du Du Baodong [Abstract] Objective To observe and establish kanamycin cochlear hair cell damage in rats in vitro model. Methods derived cochlea separation technology, in vitro organ culture techniques cochlear hair cell stereocilia and quantitative fluorescence staining was observed with different concentrations and different time of kanamycin administration on neonatal rat cochlear hair cell damage. The results of kanamycin on cochlear hair cell damage increased with the increasing dose levels, cochlear outer hair cell damage hair cells in the first place, and its lesions at the end of law is back from the cochlea to the apex development. Conclusion kanamycin cochlear hair cells in vitro dose curves and the damage on the law of the same injury in vivo. [Keywords:] hair cells, kanamycin, organ culture At present, for ototoxicity of aminoglycoside antibiotics is more gentamicin, kanamycin ototoxicity in vivo experimental data of more rarely in vitro. Vitro culture conditions can be controlled in single factors on the culture medium of cells of the inner ear, with research value. In order to explore conditions in vitro kanamycin ototoxicity experimental methods, and in the establishment of kanamycin medium damage cochlear hair cells experimental model, this study cochlear microdissection technique (1), cochlear organ culture techniques (2), the hair cell stereocilia staining technique (3,4), successfully simulated in the medium with different concentrations of kanamycin diseases caused by various degrees of cochlear hair cell damage model for further study mechanism of kanamycin ototoxicity and effective protection measures experimental basis. 1 Materials and methods 1.1 48 experimental animals were born healthy big 3 d neonatal rat, Jilin University, Laboratory animals, weight 10 g, either male or female. Animal quality cer