Kangai Astragaloside injection method for the determination Research.docVIP

 PAGE \* MERGEFORMAT 8 Kangai Astragaloside injection method for the determination Research Authors: Zhao Hongfeng, solve health Xu, Han Dong, SI Yun-shan, Xu Yajuan [Abstract] Objective: To establish Kangai Astragaloside injection method for the determination. Methods: The high-performance liquid chromatography, using Hypersil C18 5μ m (4.60 * 250 mm) column; acetonitrile - water (32:68) as mobile phase; evaporative light scattering detector; a flow rate of 1.0 mL / min. RESULTS: astragaloside 6.36 ~ 14.84 μ g in between and the peak area showed good linear relationship (r = 0.9992), the average recovery was 98.0%. Conclusion: This method is simple, accurate, reproducible, and can be used for quality control. [Keywords:] Kangai injection Astragaloside high-performance liquid chromatography Kangai injection from the astragalus, ginseng, medicines taste Oxymatrine 3 composition, the use of modern extraction and separation technology, refined traditional Chinese medicine made of injection, with Qi righting, enhance the efficacy of immune function in clinical practice for primary liver cancer, rectal cancer, malignant lymphoma, gynecologic oncology, as well as low white blood cells caused by a variety of reasons and to reduce disease and chronic hepatitis B treatment. Fang Astragalus King and medicine, astragaloside one of its main active ingredient, we use high-performance liquid chromatography Kangai Injection astragaloside, as the preparation of quality control indicators, as follows. An experimental material 1.1 Instrument Shimadzu 2010C High Performance Liquid Chromatography (Japan); Alltech 2000ES evaporative light scattering detector (United States); N2000 chromatography data workstation (Zhejiang University); AG245 Electronic Analytical Balance (Switzerland); KQ  250B Ultrasonic Cleaner (Kunshan Ultrasonic Instrument Factory). 1.2 reagent acetonitrile (HPLC pure, the United States Fisher; methanol (AR, Beijing Chemical Plant 20,070,208);