Jianpihuayu side of human hepatocellular carcinoma cells ras - MAPK pathway.docVIP

 PAGE \* MERGEFORMAT 10 Jianpihuayu side of human hepatocellular carcinoma cells ras / MAPK pathway [Abstract] Objective jianpihuayu side of human hepatocellular carcinoma cells ras / MAPK signaling pathway gene expression. Methods RT PCR method, compared with the gel electrophoresis system gene actin gene and the ratio of the absorbance reflect gene expression. Results ERK, SOS1, c fos gene expression decreased (P lt;0.05), MEK, raf, ras, Grb2 was no significant difference in gene expression (Pgt; 0.05). Conclusion jianpihuayu can only inhibit ras / MAPK signaling pathway on ERK, SOS1, c fos expression, which restrain the growth of liver cancer cells and induce apoptosis. [Keywords:] jianpihuayu side, RT PCR, ras / MAPK [Abstract] Objective To study the effect of Jianpi Huayu Recipe on ras / MAPK signaling pathway of human hepatoma cell.Methods The gene expression was represented by absorbance ratio of target gene and actin gene with RT PCR method and gel electrophoresis analysis system.Results ERK, sos1, c fos gene expression were lower, while there was no significant difference in MEK, Raf, Ras, Grb2 gene expression.Conclusion The Jianpi Huayu Recipe can inhibits the growth of the human hepatoma cell and induce the apoptosis by inhibiting the expression of gene ERK, sos1, c fos of the ras / MAPK signaling pathway. [Keywords:] Jianpi Huayu Fang; RT PCR; ras / MAPK Cellular response to extracellular stimuli through intracellular signaling pathway to achieve. MAPK is a special kind of cell signaling proteins, the role of the upstream kinases occurs after threonine, serine phosphorylation of double-point activation [1]. Is Signal transduction from the cell surface to the nucleus within the major transmitters [2], in mediating the growth, development, division, differentiation, death and the synchronization between cells and other cellular processes. the signaling pathway of basic steps to follow the MAPKs in signal transduction The three enzymat