Irregular antibody screening before blood transfusion clinical significance of.docVIP

 PAGE \* MERGEFORMAT 5 Irregular antibody screening before blood transfusion clinical significance of [Keywords:] Blood Transfusion; irregular antibodies; screening Under normal circumstances do not exist in the blood of irregular antibodies, pregnancy or repeated blood transfusion in patients with antibodies to produce red blood cells, is caused by autoimmune hemolytic transfusion reaction and cause the main difficulties with blood. Therefore, in the blood serum of patients before irregular antibodies in the screening, for the protection of blood transfusion safety and to avoid transfusion reactions is very important. now our hospital in June 2007 to December 2009 made between irregular antibody screening results reported below. 1 Subjects and methods 1.1 The study will be from June 2007 to December 2009 period, our hospital and outpatient total of 9568 cases of pre-transfusion patients as research subjects. 1.2 Reagents microcolumn gel card, centrifuge and incubation box (Changchun Bo Xun), screening cells, antibody identification of cells with a spectrum provided by the Shanghai Blood Center. 1.3 Micro-column gel card method of the three micro-column holes were added , and of 0.8% antibody screening cells in the 50l. In three holes in each micro-column by adding recipients of plasma (serum) 50l, the card into the box and incubated at 37 for 15min, centrifuged 5min, judge the results. agglutination positive results to appear, and further positive cells and patients with a spectrum of serum antibodies in the gel identification card to do. 2 Results 9568 cases were detected in 52 cases of CCP antibody positive rate was 0.54%. Which Rh system antibodies in 27 cases, accounting for 51.92%, other systems in 9 cases, accounting for 17.31%. The distribution of antibody specificity, see Table 1. Table 1 antibody screening Cha-positive cases 3 Discussion Irregular antibody screening before blood transfusion is one of the important chec