Leech extract on human hepatoma HepG2 cells in vitro study_0.docVIP

 PAGE \* MERGEFORMAT 12 Leech extract on human hepatoma HepG2 cells in vitro study [Abstract] Objective To study the leech extract on human hepatoma HepG2 cells in vitro. Methods Preparation of liquid nitrogen freeze-thaw quickly leech extract, the conventional method of water extraction and alcohol precipitation for the control of leech extract, different concentrations of human intervention in vitro hepatoma HepG2 cells, MTT assay two kinds of leech extract on HepG2 cell proliferation and cell count, acridine orange fluorescence staining to detect cell apoptosis. The results of leech extract liquid nitrogen rapid freezing and thawing method of drug concentration for the 6 ~ 15 mg / mL, the human hepatoma HepG2 cells was significantly inhibited, cell number decreased in a dose dependent manner, with water extraction and alcohol precipitation method extracts, the difference was statistically significant (P lt;0.01). acridine orange staining, typical apoptotic morphological features. Conclusions Rapid liquid nitrogen freeze-thaw method leech extract can inhibit the in vitro proliferation of HepG2 cells and induced apoptosis, and its role is better than the water extraction and alcohol precipitation Leech Law extracts. [Keywords:] hepatoma HepG2 cells, leech extract, proliferation, apoptosis, rapid freezing and thawing method of liquid nitrogen Abstract: Objective To study the inhibition effect of leech extract on HepG2 cells. Methods Human hepatocellular cancer cell line HepG2 were treated with different concentrations of leech extracts which were extracted by method of freeze-thawing with liquid nitrogen and contrasted with that by method of water extracting and ethanol precipitating. The inhibition effects and cell morphous were examined by MTT assay and Acridine orange (AO) fluorescent staining method respectively. Result The 6 ~ 15 mg / mL drug concentrations of leech extract by method of freeze-thawing with liquid nitrogen had an obvious inhibition