Liquid chip technology and application of the principle of.docVIP

 PAGE \* MERGEFORMAT 16 Liquid chip technology and application of the principle of Abstract Liquid-phase chip is the mid-20th century, 90 developed post-genomic era has been hailed as a new chip technology, with high-throughput, flexible, fast, accurate, reproducible, easy operation and many other advantages, can be used as protein and nucleic acids of biological molecules such as high-throughput detection platform. This article briefly describes the principles, advantages, and the technology in basic research, clinical diagnosis, etc. Progress in the Application are reviewed. Keywords: liquid-phase silicon; Luminex; xMAP Technology 【Abstract】 Suspension array, a novel and powerful post  genomic era tool, was first developed in 1997 based on Luminex’s xMAP technology. Featuring many remarkable advantages such as high  throughput, flexibility, rapidness and multiplexing ability, suspension array provides an open platform for assays of protein, nucleic acid and biomolecular interaction analysis. Here we provided brief introduction of its principle and advantages, and reviewed applications of this new array technology in basic research and clinical diagnosis. 【Keywords:】 suspension array; Luminex; xMAP technology Liquid chip, also known as microspheres suspended chip (suspension array, liquid chip), is based on the xMAP (flexible Multi  Analyte Profiling) technology, a new bio-chip technology platform, which is coded in different fluorescent microspheres on the antigen  antibody, enzyme  substrate  receptor ligand binding reaction and nucleic acid hybridization reaction, through the red and green laser beams were used to detect coding and reporting of fluorescent microspheres to achieve qualitative and quantitative purposes, a reaction to be completed over hole up to 100 different types of biological reactions, is the second gene chips, protein chip after the new generation of high-throughput molecular detection technology