单细胞光纳米光电检技术际球介--V1020.pptVIP

单细胞纳米光电同步检测技术 在生命科学领域的应用;;We all start out as a single totipotent cell that is programmed to produce a multicellular organism. How do individual cells make those complex developmental switches? How do single cells within a tissue or organ differ? how do they coordinate their actions or go astray in a disease process? Why do seemingly identical cells respond differently to a drug?;Single-nucleus sequencing; 单细胞转录组学分析揭示不同单细胞基因表达的巨大差别: Alex等利用脂多糖(LPS)刺激髓系来源的树突状细胞(BMDCs)，观察到单细胞在mRNA表达丰度和剪切模式方面的巨大差别，鉴定了137个刺激应答基因。 (Alex, et al. Nature, 498, 2013); 单细胞组学分析（Single-cell “omics” analysis）;Single-cell analysis heralds a new era that allows omics analysis, notably genomics, transcriptomics, epigenomics and proteomics at the single-cell level. It enables the identification of the minor subpopulations that may play a critical role in a biological process of a population of cells, which conventionally are regarded as homogeneous. It provides an ultra-sensitive tool to clarify specific molecular mechanisms and pathways and reveal the nature of cell heterogeneity. It also facilitates the clinical investigation of patients when a very low quantity or a single cell is available for analysis, such as noninvasive prenatal diagnosis and cancer screening, and genetic evaluation for in vitro fertilization. Within a few short years, single-cell analysis, especially whole genomic sequencing and transcriptomic sequencing, is becoming robust and broadly accessible, although not yet a routine practice. ; 研究单细胞分析的论文调查: 据不完全统计：2013年发表在Cell上的关于Single Cell Analysis的论文有47篇，发表在Nature上的有8篇，发表在Science上的有4篇。 本图数据是根据Science Direct数据库中生物、医学、药理学等相关领域内有关单细胞分析近五年来的论文数量统计得来。 单细胞研究成为热门方向。;多学科技术综合 采用独立开发的光,机,电,生,化一体传感技术。 可检测单个活细胞 实时检测单细胞内代谢过程中生化指标变化。 亚细胞水平光电同步检测 可在纳米尺度定位定量检测亚细胞及分子水平的光/ 电信号。 给药和药效检测同时进行 可外部给药,同时检测药物疗效。 微创检测 能够不破坏细胞结构，不损伤细胞来实现对活细胞内多种信号分子、生物标志物、酶活性、信号传递等的实时和原位检测。;产品介绍 ;Outlook;Structure;Working Principle;;电学检测：纳米探针靠近(或者刺入)待测细胞，通过工作电极检测待测目标物质的电流信号。根据浓度-电流校准曲线，可以推算出细胞所