Melatonin replacement therapy on learning and memory in rats after pinealectomy and the lateral ventricle subependymal zone changes in neural stem cell proliferation.doc

 PAGE \* MERGEFORMAT 20 Melatonin replacement therapy on learning and memory in rats after pinealectomy and the lateral ventricle subependymal zone changes in neural stem cell proliferation Guo Ling △ , lindane, Cengqing Tang, Deng-fa, CUI Wei-Gang, Wei Li, grinding Jie-lin, Lan-Ling [Abstract] Objective To observe the melatonin replacement therapy on learning and memory in adult rats pinealectomy and the lateral ventricle subependymal zone (SVZ) neural stem cell proliferation. Methods 30 healthy male Sprague-Dawley rats were randomly divided into sham surgery, pinealectomy and melatonin replacement therapy in three groups of 10 rats. In the establishment of animal models began two days of melatonin (200μ g/kg body weight) or 5% ethanol - normal saline to intervene in a fixed time each day to give each intraperitoneal injection of a second, consecutive administration for 21 days. In each group were taken at the 16th day after Morris water maze with the determination of 5 consecutive days of learning and memory ability in rats, using immunohistochemistry to observe the SVZ of the proliferating cell nuclear antigen (PCNA)-positive cells change. Results pinealectomy rats swimming in the Morris water maze escape latency and swimming distance in the former platform quadrant or decrease in the percentage of significantly longer (P amp;lt;0.01). Pinealectomy rat SVZ of the number of PCNA-positive nuclei also decreased significantly (P amp;lt;0.01). Melatonin replacement therapy can significantly reversed the changes in these indicators, and make it closer to the level of sham-operated rats (P amp;lt;0.01). Sham-operated group and melatonin replacement therapy in the indicators between the two groups was no significant difference (Pamp;gt; 0.05). Conclusion This study is the first observation, to the pineal gland makes melatonin to reduce the body can lead to learning and memory function and SVZ neural stem cell proliferation appears similar to a clear downward trend