MHCC97 in the marginal group of cells into the tumor and its invasive observation of.docVIP

 PAGE \* MERGEFORMAT 7 MHCC97 in the marginal group of cells into the tumor and its invasive observation of Author: Zhang Yi, DOU Ke-feng, SONG Wenjie, Huo Bin Liang, Zhang Fuqin [Abstract] Objective: To isolate and hepatocellular carcinoma cell line MHCC97 marginal groups in cells and to observe the tumorigenesis and invasion. Methods: Using flow cytometry fluorescence activated sorting method will be divided into the edge of hepatocellular carcinoma cell line MHCC97 group (SP) and the main group (MP) cells, two sub-groups. For two sub-groups cells were soft-agar colony forming assay and the nude mice experiment to observe its tumorigenicity in vitro and in vivo capacity; Transwell chamber assay two sub-groups in vitro invasiveness . Results: SP cells in vitro colony formation rate was 57%, MP cells, only 13%; 1 * 103 SP cells can be formed after 4 wk obvious subcutaneous tumors (2 / 8), MP cells Ze Xu 1 * 105 in order to tumor formation (2 / 8). Transwell invasion and experimental results show, SP phenotype cells through artificial basement membrane of the cell number (66.6 ± 4.0) months, MP phenotype cells only (18.2 ± 1.9) months. Conclusions: The hepatocellular carcinoma cell line in the SP subsets MHCC97 tumorigenicity and invasive subgroups are stronger than MP cells, indicating that SP phenotype of the cells in hepatocellular carcinoma growth and metastasis has an important position. [Keywords:] hepatocellular carcinoma; marginal groups; cancer stem cells; invasion 0 Introduction At present on stem cells and tumor cells of the relationship between research shows that: Cancer is a small group of stem cells with the characteristics of tumor cells that the cancer stem cells (cancer stem cell, CSC) [1] caused. CSC in tumor formation, growth, as well as infiltration and transfer plays a decisive role. We use flow cytometry fluorescence-activated sorting of hepatocellular carcinoma cell line MHCC97 Edge Group (SP) sub-group and main group (MP) sub-grou