Mercury ion based thymine thymine structure and Au amplification of mercury ion sensor.docVIP

 PAGE \* MERGEFORMAT 14 Mercury ion based thymine thymine structure and Au amplification of mercury ion sensor Study: Yang Chao Chen Zifeng Mo Zhihong Yang Linling [Abstract] Hg2 + and the use of DNA in thymine (T) with high specificity and Au in the quartz crystal microbalance (QCM) on the signal amplification, designed a simple and sensitive detection method for Hg2 +. Nanoparticles of gold with lemon sodium reduction method, the surface of the end of the oligonucleotide with thiol self-assembled modified probe, and 1 with 6-mercapto alcohol had (MCH) partial replacement of surface probe, in order to reduce steric hybridization. The results show that few nucleotide chain length 9bp, T 7 sequence number has high sensitivity, linear range of 5.0 ~ 100 nmol / L, detection limit is 2.0 nmol / L, Ca2 +, Mg2 + and other metal ions did not significantly interfere . for environmental water samples in the determination of Hg2 +, RSD [Keywords:] mercury ions, oligonucleotides, gold nanoparticles, quartz crystal microbalance Abstract A simple and sensitive method was developed for the detection of mercury ions with quartz crystal microbalance (QCM), based on the specific thymine (T) Hg2 + T structure and gold nanoparticles. The gold nanoparticles were prepared by the citrate reduction method. In a self assembled way, the particle surface was modified with the probe oligonucleotides which were partially replaced by 6 Mercaptohexan 1 ol to reduce the steric hindrance of hybridization. The sensitivity was optimized by the oligonucleotide with a strand length of 9bp and a T number of 7. The linear range was 5.0-100 nmol / L with a detection limit of 2.0 nmol / L. And Ca2 +, Mg2 + and other metal ions had no significant interferences. This method was successfully applied for the detection of Hg2 + in environmental water samples, with RSD less than 2.9% and recoveries of 97.3% -101.2%. Keywords Mercury ion, oligonucleotide, gold nanoparticle, quartz crystal micr