Membrane purified polysaccharide of Angelica sinensis.docVIP

 PAGE \* MERGEFORMAT 9 Membrane purified polysaccharide of Angelica sinensis Study: Fan Qin, Li Ying Dong, CLAY MINERALS, King Ming [Abstract] Objective To study the ultrafiltration purification process Angelica polysaccharides. Methods of different molecular weight retention membrane ultrafiltration Angelica polysaccharide extract, and the operating parameters of the orthogonal design to optimization of Angelica polysaccharide film for the detection of targets separation process conditions. The results were used angelica polysaccharide 200,100,50,20 kD molecular weight ultrafiltration membrane separation. ultrafiltration before the polysaccharide content of 30.47%, after the 200 kD ultrafiltration polysaccharide content below the highest, 65.42%. testing to determine ultrafiltration operating conditions: plus 1 times the amount of water, the operating temperature of 35 ℃, operating pressure of 0.3 MPa. Conclusion The process is simple and feasible, the resulting high purity of Angelica polysaccharide. [Keywords:] Angelica polysaccharides, ultrafiltration, purified, orthogonal Abstract: Objective To isolate and purify polysaccharide from Angelica sinensis by ultrafiltration technology. Methods Extract of polysaccharide of Angelica sinensis was ultrafiltrated with different aperture membrane, the separation parameters on membrane separation efficiency of polysaccharide of Angelica sinensis were optimized by and orthogonal test. Results Polysaccharide of Angelica sinensis was separated with 200, 100, 50, 20 kD membrane. The contents of polysaccharide was 30.47% before ultrafiltration. The main component of Angelica sinensis polysaccharide was the fraction with 200 kD (65.42%). The optimum condition of ultrafiltration was obtained as follow: one times of extract volume, 35 ℃, 0.3 MPa. Conclusion The technology was simple and available, and the content of Angelica sinensis polysaccharide was higher. Keywords:: Angelica sinensis polysaccharide, ultraf