Microbubble ultrasound on endothelial progenitor cells proliferation apoptosis and cell cycle.docVIP

 PAGE \* MERGEFORMAT 13 Microbubble ultrasound on endothelial progenitor cells proliferation apoptosis and cell cycle Study: Fan Guofeng, Feng Yi, Tong Jiayi, Yang Fang, Gen-Shan Ma, Yao Yuyu [Abstract] Objective: In vitro microbubble ultrasound on endothelial progenitor cell proliferation, apoptosis and cell cycle, in order to provide a scientific basis for further in vivo studies. METHODS: In vitro extraction, isolation and culture of porcine bone marrow progenitor cells were divided into control group, ultrasound group of ultrasound group, 24 h after the intervention of CCK 8 were used to detect cell proliferation, PI staining flow cytometry analysis of cell necrosis and apoptosis of the cell cycle. Results: (1 whispered intensity ultrasound (0.25 1.0 W cm-2 under the combined effect of micro-bubble activity of endothelial progenitor cells had no effect, higher sound intensity (gt; 1.0 W cm-2 under the effect of simply repeating what strength increased, decreased cell proliferation. (2 and control group of ultrasound group (frequency of 1 MHz, output power of 1.0 W cm-2, 90 s radiation necrosis and apoptosis of endothelial progenitor cells had no effect on cell cycle, while the ultrasound group under the same conditions, necrotic cells increased significantly, while significantly inhibited DNA synthesis. Conclusion: 1.0 W cm-2 intervention ultrasound and microbubble activity of endothelial progenitor cells on cell proliferation, apoptosis, necrosis and no significant adverse effects on cell cycle for further applications of ultrasound in vivo intervention Transplantation of progenitor cells may provide. [Keywords:] Ultrasound; microbubble progenitor cells, proliferation, apoptosis, cell cycle Drug therapy and revascularization technology continues to evolve, the mortality of acute myocardial infarction was significantly reduced, but heart failure after myocardial infarction no effective treatment. Progenitor cells (endothelial progenitor cell