Mistletoe alkaloids by liver cancer cell line P53 pathway in the research SMMC7721.docVIP

 PAGE \* MERGEFORMAT 11 Mistletoe alkaloids by liver cancer cell line P53 pathway in the research SMMC7721 Of: Guo Yanjie Zhou Qing Zhang Zhiyan Zhoucheng Jiang Fan Club [Abstract] Objective: To investigate the mistletoe alkaloids on liver cancer cell line SMMC 7721 P53 gene expression. METHODS: MTT method mistletoe alkaloids on human hepatoma cell line SMMC-7721 proliferation, semi-quantitative RT- PCR determination of P53 mRNA expression of tumor suppressor genes. Results: After the role of mistletoe alkaloids SMMC-7721 cells showed significant growth inhibition in a dose and time dependent manner. RT-PCR detection of SMMC-7721 cells, the expression of P53 mRNA relative intensity of the mistletoe alkaloids found 0.100mg/mL effect on SMMC-7721 cells after 24 hours, P53 mRNA expression increased the relative intensity (P lt;0.01). Conclusion: The mistletoe alkaloids on the expression of tumor suppressor gene P53 may be the molecular basis of inhibition of tumor growth. [Keywords:] mistletoe alkaloids, P53, RT-PCR, human hepatoma cell line SMMC-7721 Abstract Objective: To examine the effects of mistletoe alkali on P53 expression in Human Hepatocarcinoma cell SMMC-7721. Methods: MTT assay was used to evaluate the inhibitory effects of mistletoe alkali on the proliferation of human Hepatocarcinoma cell SMMC-7721. RT-polymerase chain reaction (RT-PCR) technique was used to evaluate P53 mRNA expression in human Hepatocarcinoma cell SMMC-7721. Results: Mistletoe alkali significantly inhibits the growth of SMMC-7721 cells in a time and dose-dependent manner. After exposure of SMMC-7721 cells with mistletoe alkali at 0.100mg/mL for 24 hours, the relative intensity of P53 mRNA expression level as determined by RT-PCR increases markedly (P lt;0.01). Conclusion: The stimulation of the expression of anti-oncogene P53 by mistletoe alkali may underlie the molecular basis for it to inhibit tumor growth Keywords: Mistletoe alkali, P53; RT-PCR, Human hepatoca