MMP-2 in retinopathy of premature children in the expression of neonatal mice.doc

 PAGE \* MERGEFORMAT 12 MMP-2 in retinopathy of premature children in the expression of neonatal mice [Abstract] Objective To determine the MMP-2 in the neonatal mouse model investigate the expression of MMP-2 in the pathogenesis of retinopathy of premature children in the role. Methods 7 days old C57BL/6J mice were placed in 75% + -2% high-oxygen environment, 5 days later in normal air environment, create high-oxygen-induced retinopathy of the premature children in a mouse model. at age 17 days rats were sacrificed and enucleated animals, detected by immunohistochemistry in retinal tissue The expression of MMP-2. Results MMP-2 in the control group and the oxygen exposure, integral optical density values #8203;#8203;were 16.33 + -4.13 and 21.12 + -6.29, the two had significant difference (t = 3.160, P lt;0.01 ). Conclusions MMP-2 may retinopathy in premature children have a role in angiogenesis. [Keywords:] retinopathy of premature children; MMP-2; retinal neovascularization Abstract: Objective This research using Oxygen Induced Retinopathy model examines the expressions of MMP-2 and inquires its contribution into retinopathy pathology of premature babies. Methods 7-day newborn C57BL/6J rats were raised in standardized incubator and exposed to (75 + -2 )% oxygen concentration for 5 days, then returned to normal air, in order to establish a rat model of retinopathy pathology of premature babies. Rats were killed on the 17th day of life. Retinas were dissected and stained by immunohistochemical method. Results IOD of MMP-2 was significantly increased 21.12 + -6.29 in the ROP group, and 16.33 + -4.13 in the control group. There was significant difference (t = 3.160, P lt;0.01) in the level of MMP-2 between ROP group and control group. Conclusions It suggests that the existence of MMP-2 in a rat model do exert some effect on the generation of retinal neovascularization of premature babies retinopathy pathology. Keywords:: retinopathy pathology of prema