Modified adriamycin rat model.doc

 PAGE \* MERGEFORMAT 15 Modified adriamycin rat model Of: Yang Weina, in Lin Hua, Guo Shang temperature, into a small profit, Chai Yun [Abstract] Objective To investigate the pathological adriamycin at different stages of development the animal model. Methods interval of 2 weeks twice a tail vein injection method of adriamycin rat model of adriamycin, rats in each group of blood, urinary biochemical parameters and renal histopathological changes. The results of the model showed severe edema, massive proteinuria, hypoalbuminemia and hyperlipidemia; pathological examination revealed swelling of 4 weeks the glomerular podocyte, mesangial cells mild hyperplasia; 8 weekend balloon adhesion, renal tubular epithelial cell atrophy; segmental glomerular part of the 12th week of mild to moderate sclerosis, there was focal interstitial lymphocytic infiltration and mild fibrosis. Conclusion The improved interval of 2 weeks twice a tail vein injection of adriamycin 4mg/kg, can be successfully replicated adriamycin. acute model of pathological changes similar to human minimal change nephropathy and chronic pathological changes similar to the model’s Bureau segmental glomerulosclerosis lesions. [Keywords:] adriamycin; foot cell; minimal change nephropathy; focal segmental glomerulosclerosis ABSTRACT: Objective To study the adriamycin nephrosis model in different development pathological stages. Methods The rat adriamycin nephrosis model was established by injecting adriamycin into tail vein twice every two weeks to detect blood and urina biochemical indicators and to observe the pathological changes of the renal tissues . Results The model showed serious edema, proteinuria, hypoproteinemia, and hyperlipidemia. Podocytes were swollen and mesangial cells developed mild hyperplasia at the end of the fourth week. The nephric tubule atrophied at the end of the eighth week accompanied with adhesion between glomeruli and Bowman s capsule. Glomeruli sclerosis of mild or