Morphine induced by cisplatin in nasopharyngeal carcinoma CNE-2 cells in nude mice transplanted tumor growth inhibition of.doc

 PAGE \* MERGEFORMAT 18 Morphine induced by cisplatin in nasopharyngeal carcinoma CNE-2 cells in nude mice transplanted tumor growth inhibition of Study: Lung Hui Zhou Jianhua Lin speaking before the Red Eagle was Vivian Tan Yixin has [Abstract] [Objective] The human nasopharyngeal carcinoma CNE-2 cells in nude mice as a model of morphine on tumor growth inhibition effect of cisplatin and its mechanism. [Method] CNE-2 cells to establish nude mouse model 20 rats were randomly divided into 4 groups, control group, cisplatin treatment group, drug group morphine alone and morphine plus cisplatin treatment group, n = 5. morphine 5 mg / kg administered 1 time per day, cisplatin 3 mg / kg administered every 3 d 2, are injected. animals were sacrificed after 14 d treatment, complete dissection of tumor, tumor weight in mind the net. TUNEL staining based on cell apoptosis, immunohistochemical staining of tumor tissue Bax, Bcl -2, cleaved-caspase-3 and CD34 expression. [results] cisplatin alone treatment group, tumor weight was significantly smaller than the other 3 groups (P lt;0.05), morphine and cisplatin treatment group, tumor weight was significantly lower than control group (P lt;0.05); TUNEL staining and cleaved-caspase-3 staining showed that cisplatin alone treatment group, the number of apoptotic cells was higher than the other 3 groups (P lt;0.05). cisplatin alone treatment group compared with the other 3 groups Bax protein expression increased, and decreased expression of Bcl-2, morphine alone group, the strongest expression of Bcl-2. CD34 staining showed microvessels cisplatin alone treatment group, microvessel density was significantly less than the other 3 groups (P lt;0.05), simple The MVD of morphine treatment group were significantly more than the control group (P lt;0.05). [Conclusion] morphine by antagonizing cisplatin-induced apoptosis and promote the CNE-2 cells in nude mice transplanted tumor angiogenesis, inhibit tumor resistance to cisplatin