Modified tissue culture needle biopsy of synovial fibroblast-like synovial cells.doc

 PAGE \* MERGEFORMAT 19 Modified tissue culture needle biopsy of synovial fibroblast-like synovial cells Study: Lie Dai Bin Zheng Donghui Li Ting Zhu Langjing Mo Yingqian Zhang Baiyu [Abstract] [Objective] To investigate the in vitro isolation and culture of fine needle biopsy of synovial fibroblast like synoviocytes (FLS) method. [Method] synovial tissue from the blind acceptance of synovial biopsy needle-type activities rheumatoid arthritis, digestive enzymes were used to culture, tissue culture and improved tissue culture method were isolated and cultured FLS. Trypan blue staining for cell count and identification of its activity, and inverted phase contrast microscope, transmission electron microscopy, immunocytochemistry , flow cytometry, 3 ~ 4 FLS were identified. [results] three kinds of methods for isolating and culturing primary can be successfully cultured FLS. trypan blue staining showed FLS cell count of about (8.30 + - 1.65) 105 / bottle, living cells was “95%. Chuandaikeshi FLS to purification on the morphological requirements of inverted phase contrast microscope and transmission electron microscope are consistent with the morphological structure of FLS, immunocytochemistry showed Vimentin , CD68 , CK Yang Xing flow cytometry showed CD55 + cells were (95.34 + - 2.47)%. FLS modified tissue culture a higher success rate, cells swim earlier and required a shorter generation time. [Conclusion] modified tissue particularly suitable for fine needle biopsy specimens of synovial FLS training, 3 to 4 the number of cells, activity and purity of line with the requirements for further experiments. [Keywords:] fibroblast-like synovial cells; cell culture; fine needle biopsy Abstract: [Objective] To explore the culture method for fibroblast-like synoviocytes (FLS) from needle biopsied synovium tissue. [Methods] Synovium tissue obtained from patients with active rheumatoid arthritis by blind needle synovium biopsy were cultured with three different