Mountain Song Songta anti-HIV activity in the determination of polysaccharide.doc

 PAGE \* MERGEFORMAT 8 Mountain Song Songta anti-HIV activity in the determination of polysaccharide Of: Zhang Zhen, Zhang Peng, Han Xiandong, Yang Min, Liu Fan, Liu Guangming [Abstract] Objective To establish pine Pinus armandii Franch. Songta the AIDS virus (HIV) Determination of polysaccharide content of the active site. Methods phenol - sulfuric acid color, in the 489.0 nm wavelength ultraviolet spectrophotometry polysaccharide content. results of the regression equation is: A = 0.011 3C-0.058 2, the correlation coefficient r = 0.999 6, the measured anti-HIV active site (Ⅰ ~ Ⅲ) polysaccharide content was: 64.69%, 67.14%, 71.85%; average recovery was 99.32%, RSD was 0.34% (n = 6). Conclusion The method is fast, simple, sensitive and can be used as anti-HIV activity of pine cone in the Determination of polysaccharide part. [Keywords:] Polysaccharide; Anti-HIV active site; pine; phenol - sulfuric acid method Abstract: ObjectiveTo determine the content of polysaccharides in the activity sites from pine cone of Pinus armandii Franch.MethodsPhenol-sulfuric acid method was used to determine the content of polysaccharides at 489.0nm.ResultsThe regression equation was A = 0.0113C-0.0582, r = 0.999 6. The contents of polysaccharides in anti-HIV active sites Ⅰ - Ⅲ was 64.69%, 67.14%, 71.85%, respectivety. The average recovery was 99.32% and RSD was 0.34% (n = 6). ConclusionThe method is rapid, reliable and sensitive, and it can be used to determine the contents of polysaccharides in the activity sites from pine cone of Pinus armandii Franch. Keywords:: Content determination; Polysaccharides; Anti-HIV; Active site; Pinus armandii Franch; Phenol-sulfuric acid method Rich genus of Songke Song, 22, 10, varieties, and the other the introduction of 16 species, and two variants, for a total of more than fifty species are very widespread [1]. The multi-species with medicinal value, as natural medicine has a long and at home and abroad a wide range of applica