Multiple cerebral infarction in rats model-making and its significance.doc

 PAGE \* MERGEFORMAT 7 Multiple cerebral infarction in rats model-making and its significance Author: car light up, Zhou Li Nan, Zhang Hui, Zhang Wei, Zhang Ye Our country is a higher incidence of cerebrovascular disease in the country in recent years, with the continuous improvement of people’s material standard of living, the aging population increases, the incidence was rising rapidly. Cerebral vascular diseases in high risk, high disability, high mortality and serious impact on human health, while the multiple cerebral infarction (Multiple Cerebral Infarction, MCI) the incidence of possession of a large proportion. So far, MCI in the pathogenesis is not entirely clear, there is no highly effective treatment, therefore, to study its pathogenesis, prevention and control measures of great significance. In this study, an animal model can be used to establish the clinical treatment of the disease conditions for provision of laboratory animal model for clinical research of new medicines to provide theoretical basis for laboratory 〔 1〕 . 1 Materials and methods 1  1 Materials male Wistar rats (Animal Center, China Medical University); LUZEX-F micro-image analyzer (Japan Panasonic Corporation); Hitachi H-600 transmission electron microscope; constant cold box slicer. 60 male Wistar rats weighing 260 ~ 300g, were randomly divided into 3 groups. Control group, 20 (10 for electron microscopy staining, 10 used for electron microscopy); MCI model light microscopy group 20; MCI model of electron microscopy group of 20. 1  2 Method 1  2  1 MCI model prepared with 1% pentobarbital sodium (40ml/kg) intraperitoneal injection of anesthesia for neck incision exposed the left common carotid artery, respectively, has to find the internal carotid artery and external carotid artery bifurcation, do pterygopalatine artery ligation, and full external carotid artery dissection. In the common carotid artery and carotid artery distal ends, respectively, injury-free micro-v