Multiple myeloma bone marrow mesenchymal stem cell biology Analysis.doc

 PAGE \* MERGEFORMAT 10 Multiple myeloma bone marrow mesenchymal stem cell biology Analysis Author: Zhu Yi Bu Dong Ren Jun Wang Xiang-Yun Li Xin Nie 【Abstract】 In order to study multiple myeloma (multiple myeloma, MM) patients with bone marrow-derived mesenchymal stem cells (mesenchymal stem cells, MSC) of the pathological biological characteristics, to explore the MSC in the MM bone lesions are the role of the 11 cases taken in early Governance MM patients and 5 cases of normal bone marrow, adhesion in vitro using isolated and cultured MSC. MM by flow cytometry analysis of bone marrow MSC phenotype, MTT assay MSC proliferation, staining cells was determined to differentiate into bone cells and fat cell differentiation. Application of enzyme-linked immunosorbent assay (enzyme-linked immunosorbent assay, ELISA) determination of cell culture supernatant of interleukin -6 (interleukin-6, IL-6) and stem cell growth factor (stem cell factor, SCF) concentrations. MSC culture supernatant collected as the conditioned medium, according to the concentration gradient by adding human SKO007 myeloma cell culture system, MTT determination of differences in cell proliferation. The results showed that: MSC compared with normal bone marrow, MM patients with bone marrow MSC phenotype no change, uniform expression of CD29, CD73, CD166, and HLA-ABC, did not express CD45 and CD31; MM patients with bone marrow MSC proliferation and differentiation to normal, and has a similar to bone and fat differentiation into functional; MM bone marrow MSC secretion of IL-6 and SCF were significantly higher than normal water; MM sources of MSC culture supernatant significantly promote SKO007 cell proliferation. Conclusions: MM patients with bone marrow proliferation and differentiation of MSC function was normal, MM, when bone injury may be related to MSC differentiation of unrelated, but high IL-6 and SCF expression in bone marrow tumor cells provide the necessary survival signals.