Nano-water extract of dogwood aging mouse brain p66shc expression.doc

 PAGE \* MERGEFORMAT 9 Nano-water extract of dogwood aging mouse brain p66shc expression Study: Lily Wang Mingfu parsley Zhugui Ming Wang Chao-cold [Abstract] Objective To investigate the preparation of the natural aging mice dogwood brain tyrosine phosphorylation signaling adapter protein (66kilodalton isoform of shcgene products, p66shc expression. Methods in captivity of natural aging in mice 36 11 to 12 months , 8 young rats, divided into 6 groups: young control group, aging control group and treatment group, treatment group was divided into water extract of dogwood and nano-alcohol extract of the high-dose and low dose groups, each group of 30 drug d, and then decapitated mouse liver tissue superoxide dismutase (SOD, malondialdehyde (MDA detection, RT PCR method p66shc expression in mouse brain. Results dogwood nano liquid water extraction and ethanol administration SOD activity and aging group was significantly higher than the control group (P lt;0.01, while the MDA content and the expression of brain p66shc significantly lower (P lt;0.01, water extract of dogwood nanometer low-dose formulations, high-dose group and compare the alcohol extract effects were more significant (P lt;0.01. Conclusion dogwood can significantly reduce the activity of brain of aging p66shc expression of subunit, thus affecting the p66shc activity play a role in delaying aging. dogwood Nano small dose of water extract of dogwood was better in the nano- alcohol extract of the group. [Keywords:] dogwood, p66shc activity, aging mice, brain tissue, the water extract of nano Phosphotyrosine signaling adapter protein (66kilodalton isoform of shcgene products, p66shc) in recent years of more genes, some animals p66shc gene knockout can be extended the life of individual organisms (1.) Study of the gene Aging and cell signal transduction linked to (2) as the aging mechanism of the new hot spot. at the molecular level, the normal aging process in the stress gene expression also