Nested MSP assay malignant hematological cell lines p16 gene promoter methylation status of the study.doc

 PAGE \* MERGEFORMAT 21 Nested MSP assay malignant hematological cell lines p16 gene promoter methylation status of the study Authors: Zhou Hua-rong, SHEN Jian-zhen, FU Hai-Ying, Ye Bao-Guo, Fan Liping, Lin Fuan Abstract In this study, an improved methylation-specific PCR (MSP) method, that is, nested methylation-specific PCR to detect six kinds of cancer cell lines p16 gene promoter methylation status of the efficiency of its screening p16 gene promoter hypermethylation of tumor cell lines, and as a study of the relationship between gene methylation and expression of an ideal cell model applications. 6 kinds of tumor cell genomic DNA after denaturation by the alkali sulfite modification, and then nested methylation-specific polymerase chain reaction amplification, analysis detected p16 promoter region CpG island methylation status. The results showed that: CA46, U266 varying degrees of p16 gene promoter methylation, but Molt4, K562, HL-60, Jurkat for p16 gene promoter were not methylated. Conclusion: The nested methylation-specific PCR can accurately detect malignant hematological cell lines p16 gene methylation status, method is simple, sensitive, and reproducible strong, can be widely used for screening of p16 gene promoter region Methylation of malignant hematological cell lines as well as the diagnosis of hematologic malignancies. Keywords: nested methylation-specific polymerase chain reaction Detection of Promoter Methylation of p16 Gene in Hematological Malignant Cell Lines by Nested Methylation Specific Polymerase Chain Reaction Abstract This study was aimed to investigate the effeciency of modified methylation-specific polymerase chain reaction ienested methylation-specific polymrase chain reaction, used to detect the promoter methylation of p16 gene in six hematological malignant cell lines, and to explore the application in selection of hematological malignant cell lines with promoter hypermethylation, and make them