Nettle root extract part of the rough water on the AA rat peritoneal macrophages in vitro PGE2 levels and peritoneal macrophages in LPS-induced COX-2 mRNA expression in.doc

 PAGE \* MERGEFORMAT 9 Nettle root extract part of the rough water on the AA rat peritoneal macrophages in vitro PGE2 levels and peritoneal macrophages in LPS-induced COX-2 mRNA expression in Authors: Xiao-Hong Li, Li Shunying, Zhaoyong Na, Li Bing-lan Shao Xiaoxia [Abstract] Objective To observe the Yunnan middle part of the crude water extract nettle root Urtica macrorrhiza Hand-Mazz, Ur of the adjuvant arthritis (adjuvant arthritis, AA) in rat peritoneal macrophages (peritoneal macrophages, PMφ ) secretion of prostaglandin E2 ( prostaglandin E2, PGE2) levels and on in vitro LPS-induced PMφ COX-2 mRNA expression, to explore its possible mechanism of treatment of rheumatoid arthritis. Methods AA rat model, Ur part of a continuous intragastric administration of water extract 14 d or 21 d after the fractionated to obtain PMφ , LPS-induced production of PGE2, using enzyme-linked immunosorbent assay detection of supernatant PGE2 levels. In vitro PMφ , RT-PCR method of detection of LPS-induced expression of COX-2 mRNA. Results Ur water extraction part (400,200 mg / kg) on LPS-induced AA PMφ significantly inhibit the secretion of PGE2 levels. Ur (200, 100 μ g / ml) significantly inhibited LPS-induced COX-2 mRNA expression. Conclusion Yunnan producing crude water extract nettle root Part of the therapeutic effect of adjuvant arthritis may be related to inhibition PMφ secretion of PGE2 and COX-2 mRNA expression. [Keywords:] coarse root Nettle adjuvant arthritis prostaglandin E2 COX-2mRNA Abstract: ObjectiveTo investigate the effects of aqueous fraction of Urtica macrorrhiza Hand-Mazz (Ur) on modulating prostaglandin E2 (PGE2) production in vivo and the expression of COX-2 mRNA in vitro and elucidate the possible mechanisms of anti-inflammatory and anti - rheumatoid effects of Ur. MethodsAdjuvant arthritis (AA) rat was used as the model. The PMφ samples were taken at different time after medication. PGE2 level was measured by ELISA method. PMφ induced by lipopo