Nettle is a plant to study the impact of bone formation.doc

 PAGE \* MERGEFORMAT 10 Nettle is a plant to study the impact of bone formation Author: Shi Liying ZHOU Yuan Tang Ling Bao-Min Feng Yong-Qi Wang Yan Xingguo 【Abstract】 Objective To observe the four kinds of nettle genus of 8 different extracts on bone formation. Methods osteoblast-like MC3T3-E1 cells as target cells to epidermal growth factor as a positive control, four kinds of nettle genus of 8 different extracts were divided into 50,100,200 μg / ml 3 different concentrations groups, using CCK -8 (cell counting kit-8) Determination of living cell count four kinds of nettle genus of 8 different extracts on osteoblast-like MC3T3-E1 cells, the proliferation of the role of simultaneous determination of alkaline phosphatase activity. Results triangular leaves alcohol nettle root extract, nettle leaf width alcohol whole plant extract and alcohol extract water layer over D101 macroporous resin, 40% of the elution part of the 50 to 200 μg / ml of concentration could significantly promote the osteoblast-like MC3T3-E1 cell proliferation. At the same time these three kinds of extracts to a certain extent, also enhanced ALP activity, but no significant difference. Conclusion triangle and wide-leaf nettle nettle leaf extract in vitro osteoblast-like MC3T3-E1 cells, suggesting the role of bone formation in vivo may contribute to the process. Keywords: nettle genus; into bone cells; proliferation; alkaline phosphatase; activity Abstract: ObjectiveTo study the effect of eight extracts of four Urtica plants on bone formation. MethodsOsteoblastic MC3T3-E1 cell was used as the target cell, epidermal growth factor was used as positive control, MC3T3-E1 cells were cultured and tested for growth with various concentrations of samples in the medium. The proliferation effect of eight extracts of four Urtica plants on clone MC3T3-E1 cells was examined with cell counting kit-8 (CCK-8) and alkaline phosphatase (ALP) activity was also determined. ResultsWe foun