Neurotransmitter substance P by regulating the expression of transcription factor Osterix promote osteoblast differentiation.doc

 PAGE \* MERGEFORMAT 15 Neurotransmitter substance P by regulating the expression of transcription factor Osterix promote osteoblast differentiation Of: Hai Biao, Liu Qiang, Guomin Feng, Hua-Ping Zhang, Chen Jun Long [Abstract] Objective To study the regulation of neurotransmitter substance P into the molecular pathways of bone cell differentiation. Isolated primary bone marrow stromal cells were cultured and subcultured, the control respectively, P substance, P substance NK1 receptor antagonist, P substance + substance P NK1 receptor antagonist intervention, induction of bone marrow stromal stem cells into bone cells, subcultured 1 to 2 weeks after the extraction of total cellular RNA, using RT PCR detection of Osterix during differentiation of gene expression. test results repeated 3 times, single factor analysis of variance test results. The results of bone marrow stromal cells in the growth of the number of proliferating period of 4 ~ 6d, using RT PCR detected P substance intervention of osteoblast differentiation, leading to osteoblast differentiation important transcription primer Osterix gene expression, compared with the other groups were significantly different (P lt;0.05), Osterix upregulated genes, thereby stimulating bone cells before the cells into osteoblasts. the substance P NK1 receptor antagonist + P co-intervention in substance, Osterix gene Expression with the control group no significant difference (P lt;0.05), that substance P NK1 receptor by substance P on the regulation of osteoblast differentiation. Conclusion substance P can regulate osteoblast differentiation prior to the process of transcription factor Osterix gene expression to promote its differentiation into osteoblasts. P substance on the regulation of gene expression relies Osterix substance P NK1 receptor. [Keywords:] P substance, osteoblasts, differentiation, Osterix ABSTRACT: Objective To study the molecular pathway of osteoblastic differentiation induced by substa