Nephritis Ning on LPS induced mesangial cell proliferation and extracellular signal-regulated protein kinase.doc

 PAGE \* MERGEFORMAT 12 Nephritis Ning on LPS induced mesangial cell proliferation and extracellular signal-regulated protein kinase Study: East side of Fan Huanfang Guodeng Zhou Yuan Guodong WANG Yue-hua [Abstract] Objective nephritis Ning on human mesangial cells (HMC) proliferation and extracellular signal-regulated protein kinase (extracellular signal regulated protein kinase, ERK). Methods Cell culture technology for HMC culture Serum pharmacological method to prepare serum nephritis rather, the use of lipopolysaccharide (LPS) for the stimulating factor, application of tetrazolium salt (MTT assay and enzyme-linked immunosorbent assay (ELISA) detection of cell proliferation and phosphorylation of ERK1 / 2 expression. Results nephritis rather significantly inhibit HMC proliferation, inhibition rate of 12,24 and 48 h were 16.0%, 15.4%, 18.2%, LPS group of phosphorylated ERK1 / 2 content was significantly higher compared with the control group ( P lt;0.05), traditional Chinese medicine group and blank group, the phosphorylation of ERK1 / 2 decreased significantly (P lt;0.05), LPS + nephritis Ning group and LPS group, the phosphorylation of ERK1 / 2 expression was significantly lower (P lt;0.05). Conclusion nephritis rather inhibit LPS stimulation of normal culture conditions and under the conditions of phosphorylated ERK1 / 2, which play a role in the treatment of glomerulosclerosis. [Keywords:] Ning nephritis, mesangial cell culture, proliferation, extracellular signal-regulated protein kinase [Abstract] Objective To investigate the effect of Shenyanning on the expression of extracellular signal regulated protein kinase of human mesangial cells (HMCs). Methods HMCs were cultured by applying cell cultural technique.The serum pharmacological method was used in the preparation of shenyanning serum. HMC proliferation was detected by MTT.The p ERK1 / 2 expression was detected by enzyme linked immunosorbentassay (ELISA) taking lipopolysaccharide (LPS) a