NFB and its related inflammatory factors induced by cold preservation injury in liver transplantation mechanism.doc

 PAGE \* MERGEFORMAT 16 NFB and its related inflammatory factors induced by cold preservation injury in liver transplantation mechanism Of: Jiang An, Liu Feng, Liu, Song Yulong, Meng Kb Wei, Lu Yi Jiang An [Abstract] Objective of liver transplantation in rats at different time of reperfusion after cold preservation of nuclear factor B (nuclear transcription factor B, NF B) and activation of inflammatory factors related to determine cold storage time, NF B activation and liver transplantation the links between injury. Methods Wistar rats were established by orthotopic liver transplantation model, the sham group (A group) as the control group, rats transplanted liver in 4 UW solution preserved 45min (B group), 120min (C group), 180min (D group) and 3h after reperfusion liver tissue, serum NF B, tumor necrosis factor-@ (TNF @), interleukin 1 (IL 1) and liver damage indicator alanine aminotransferase ( ALT), aspartate aminotransferase (AST) and changes in liver cells. Results of liver transplantation with extended cold preservation time gradually NF B activation (P lt;0.05), and to the liver tissue TNF @, IL 1 increased levels (P lt;0.05), after reperfusion, NF B activation taking a step forward (P lt;0.05), liver tissue TNF @, IL 1 further increased (P lt;0.05), ALT, AST and liver cell apoptosis index was significantly rise higher (P lt;0.05), increased liver damage. Conclusion NF B in the liver of the cold storage period was extended over time induced activation of the explosive after reperfusion was activated, and raised its target genes by TNF @, IL 1 The transcription of liver damage, which may be the liver cold preservation and reperfusion injury an important mechanism. [Keywords:] liver transplantation, organ preservation and cold preservation and reperfusion injury, nuclear factor B ABSTRACT: Objective To establish a model of rat orthotopic liver transplantation and investigate the relationship among cold preservation time, activation of nuclea