Of a hypoglycemic compound Chinese herbal medicine extract Determination of total polysaccharides.docVIP

 PAGE \* MERGEFORMAT 8 Of a hypoglycemic compound Chinese herbal medicine extract Determination of total polysaccharides Author: Gao Wei, Xiao-Juan, Liu Yi, Du Lian-Xiang [Abstract] Objective To establish a spectrophotometric determination of hypoglycemic compound traditional Chinese medicine extract the content of total polysaccharide. Method of ultraviolet spectrophotometry, phenol - sulfuric acid method. Results The linear range of 5 ~ 40 μ g / ml, r = 0.999 3, the average recovery was 99.36%, RSD = 3.15%. Conclusion The method is simple and good reproducibility. [Keywords:] compound hypoglycemic medicine extract; total polysaccharides; UV spectrophotometry; determination From Astragalus, litchi nuclear, hawthorn leaves and seeds of Leucaena mixed according to a certain percentage, after certain processing of the extraction process extracts obtained by pharmacological experiments show that with good reduces blood sugar, blood lipid regulating effect. Polysaccharides, one of its main active ingredient. In this paper, UV spectrophotometry, phenol - sulfuric acid method for the extraction of the total polysaccharide content in the determination of quality standards for its research and development of indicators to provide experimental basis. Results show that the method is simple and sensitive and fast-easy, can be used to control the product quality. An instrument and reagent 1.1 Instrument nucleic acid protein analyzer Reid from Guangzhou Bio-Technology Co., Ltd. production. 1.2 hypoglycemic drug test compound traditional Chinese medicine extract from the hospital on their own making; Glucose Standard: Inspection of Pharmaceutical and Biological Products Chupin; concentrated sulfuric acid, phenol, ethanol were of analytical grade. 2 Methods and Results 2.1 Preparation of glucose standard solution precision Weigh 105 ℃ dried to constant weight anhydrous glucose standard 5 mg, buy 100 ml flask, add distilled water to dissolve and dilute the scale, sha