Of estrogen on the ability of normal sperm and its mechanism of.docVIP

 PAGE \* MERGEFORMAT 20 Of estrogen on the ability of normal sperm and its mechanism of Author: Yan-Fang Li-Min Yue Zheng Ya-ping Zhang Jinhu 【Abstract】 Objective To study the material of estrogen 17β-estradiol (E2) on the normal reproductive ability of men, in vitro fertilization, and to explore its possible mechanism. Method of sperm suspension were added in different concentrations of E2 and non-transparent membrane Crosslinked material Estradiol - bovine serum albumin (E2-BSA), was able to 4h Across vitro fertilization was observed egg fertilization rate (FR) and fertilization index (FI) changes; and further removal of medium calcium ion (Ca2), once again observe the non-calcium environment of E2 on human sperm effects of FR and FI. The results of a certain concentration of E2 (1μmol / L, 10μmol / L) can significantly improve human sperm FR and FI (P lt;0.05), lower and higher concentrations of E2 (0.1μmol / L, 100μmol / L) then the right person sperm FR and FI had no significant effect (Pgt; 0.05); In addition, non-transparent membrane macromolecules E2-BSA (2mg / L) can significantly improve human sperm FR and FI (P lt;0.05). Have been able to remove the liquid in the Ca2 after, E2 on human sperm FR and the FI had no significant effect (Pgt; 0.05). Conclusion culture medium by adding a certain concentration of E2 can improve human sperm in vitro fertilization rate, and its mechanism may be through with the sperm membrane estrogen receptor or estrogen-interacting binding sites for the extracellular Ca2 be achieved within the current The. 【Key Words】 17β-estradiol; Estradiol - bovine serum albumin; human sperm; fertilization; hamster oocyte Abstract: Objective To study the effect and possible mechanism of 17β-esrtrodial (E2) on the fertilizing ability of human spermatozoa in vitro.Methods Spermatozoa were exposed to E2 at different concentrations and the membrane impermeable conjugated 17β-estradiol (E2-BSA) respectively . After