Of iron deficiency on rat gastric emptying and its mechanism.docVIP

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2017-05-05 发布于浙江
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Of iron deficiency on rat gastric emptying and its mechanism.doc

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Of iron deficiency on rat gastric emptying and its mechanism

Of iron deficiency on rat gastric emptying and its mechanism Keywords:: iron-deficiency anemia gastric physiology reason animal model of gastrointestinal diseases Abstract Objective: To study the function of iron deficiency on rat gastric emptying and its mechanism. Methods: Wistar rats were 4wk age of 60 were randomly divided into two groups: iron-deficiency anemia (IDA) group and normal control group, all 30 only, respectively, are fed with low iron and iron diet (iron content, respectively 12.4mg/kg and 385.5mg/kg), a total of 8wk. emptying test using phenol red determination of gastric emptying to calculate evacuation rate; by DCP - AES determination of the organization to measure the iron content antrum; RIA determination of antral tissues content of cyclic nucleotides cAMP and cGMP; staining showed antral myenteric plexus AchE and NOS, and application of color image analysis system measured positive specimen object plane density. Results: IDA gastric emptying rate in rats (90 ± 10)%, significantly higher than the control group (78 ± 9)% (P amp;lt;0.05); Organization of iron content antrum (30 ± 9) μ g / g , significantly lower than the control group (311 ± 69) μ g / g (P amp;lt;0.01); gastric tissue supernatant of cAMP and cGMP concentrations were (11.7 ± 3.8) nmol / L and (2.0 ± 0.7) nmol / L, were was significantly lower than the control group (15.0 ± 2.6) nmol / L (P amp;lt;0.05); (2.8 ± 0.4) nmol / L (P amp;lt;0.01), but the cAMP / cGMP ratio (5.8 ± 0.2), significantly higher ( 5.4 ± 0.1) (P amp;lt;0.01); antral myenteric plexus AchE areal density (11.8 ± 1.2)%, significantly lower than the control group (13.9 ± 1.6)% (P amp;lt;0.01), but the NOS surface density (10.8 ± 1.6)%, significantly higher than that (9.5 ± 1.0)% (P amp;lt;0.05). Conclusions: IDA hyperthyroidism gastric emptying in rats. deficiency may influence the Ach-cAMP, NO-cGMP information transmission, so that cAMP / cGMP ratio increased significantly, resulting in gastric smo