Of ketamine on myocardial ischemia-reperfusion myocardial cell apoptosis and Fas protein Bcl.docVIP

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2017-05-05 发布于浙江
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Of ketamine on myocardial ischemia-reperfusion myocardial cell apoptosis and Fas protein Bcl.doc

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Of ketamine on myocardial ischemia-reperfusion myocardial cell apoptosis and Fas protein Bcl

Of ketamine on myocardial ischemia-reperfusion myocardial cell apoptosis and Fas protein Bcl [Keywords:] myocardial ischemia-reperfusion injury;,, myocardial apoptosis;,, ketamine;,, Fas protein;,, Bcl Abstract: Objective: To investigate the role of ketamine in rats under experimental myocardial ischemia-reperfusion myocardial cell apoptosis and Fas and Bcl2 protein expression changes and the relationship between myocardial tissue injury, and pathological analysis of myocardial injury. Methods: threading Preparation of the left coronary artery ligation or Sonza myocardial ischemia and reperfusion model. 32 rats were randomly divided into sham operation group (sham 4.5 h), ischemia-reperfusion group (ischemic 30min, reperfusion 4 h), low-dose ketamine ischemia-reperfusion group (ischemic 30min, reperfusion 4h) and High-dose ketamine in ischemia-reperfusion group (ischemic 30min, reperfusion in a 4 h). With nick-end labeling to detect changes in myocardial apoptosis, SP immunohistochemical method were used to detect Fas and Bcl2 protein levels change, it is pathological biopsy of myocardial injury. Results: Myocardial ischemia-reperfusion myocardial apoptosis index and Fas protein staining index and Bcl2 protein staining index increased, ketamine can reduce myocardial apoptosis, reduction of Fas and Bcl2 protein expression of positive cells; myocardial ischemia after reperfusion showed different sizes of the focal myocardial necrosis, necrosis surrounded by inflammatory cell infiltration, necrosis, after effects of ketamine reduced the role of low doses of ketamine is more obvious. Conclusion: Myocardial ischemia-reperfusion myocardial cell apoptosis, Fas gene protein and Bcl2 protein expression increased volume, ketamine can reduce myocardial apoptosis, reducing cell Fas and Bcl2 protein expression, thereby reducing myocardial injury, and low dose of ketamine effect more pronounced. Keywords:: myocardial ischemia-reperfusion injury; myocardial cell apoptosis; ke