On High Performance Liquid Chromatography after drinking the contents of hesperidin_0.docVIP

 PAGE \* MERGEFORMAT 15 On High Performance Liquid Chromatography after drinking the contents of hesperidin [Abstract] Objective To establish drinking after the determination of hesperidin. Methods using high performance liquid chromatography column was CenturySIL C18-AQ (250 mm × 4.6 mm, 5 μm), mobile phase of acetonitrile -0.2% phosphoric acid (21:79), flow rate 0.8 mL / min, detection wavelength was 284 nm, column temperature was at room temperature, the injection volume was 20 μL. Results of hesperidin concentration range of 1.004 ~ 10.04 μg good linear relationship, the average recovery was 98.59%, RSD = 0.72%. Conclusion The method is simple, accurate, reproducible and can be used as drinking in the operation method for the determination of hesperidin. [Key Words] high performance liquid chromatography, after drinking, hesperidin, determination Keywords:: HPLC, Shuhouyin, hesperidin, content determination Department of Wuwei drinking after the development of hospital pharmacy Cancer Hospital, is heterophylla, diffusa, dried tangerine peel, licorice, etc. Smell the mixture made with qi qi, qi and wide, the Jiangni functions under the gas, used after surgery due to blood deficiency in the treatment of bowel gas barrier Zhu Zheng. After many years of clinical use, a significant effect. to control the quality of reference [1-6], high performance liquid chromatography (HPLC) method in the other Citrus herbs the main active ingredient content of hesperidin were determined, the result is simple, accurate, reproducible and after drinking quality standards for the research and development of indicators to provide an experimental basis. An instrument and reagent SSI PC-2000 high performance liquid chromatography, Model500 UV detector, 7725i manual injection valve, of Star chromatography workstation, XT120A electronic analytical balance (瑞士普利赛斯), HS10260D ultrasonic cleaner (Austrian Science and Technology, Tianjin Heng Development Limited). H