On the GFW tumor-bearing mice on tumor cell apoptosis.docVIP

 PAGE \* MERGEFORMAT 13 On the GFW tumor-bearing mice on tumor cell apoptosis Paper Keywords: GFW; apoptosis; Survivin mRNA Abstract: Objective: To explore the GFW-induced tumor cell apoptosis mechanism for GFW (GFW) to provide experimental basis for development and application. Methods: The calculation of inhibition rate, apoptotic rate detected by flow cytometry, electron microscopy ultrastructure of tumor cells. In situ hybridization to detect expression of Survivin mRNA. Results: GFW tumor inhibition rate was 38.93%, flow cytometry GFW apoptosis rate 17.79%, compared with model group, the difference was statistically significant. GFW Group microscope, the main changes in the tumor cells to apoptosis. Endometrial structure intact, membrane clear, cell shrinkage, chromatin Mission massive dispersal of nuclear membrane within or under the set of edges, and apoptotic bodies can be seen. GFW down Survivin mRNA expression in tumor cells, compared with model group, the difference was statistically significant. Conclusion: GFW-induced tumor cell apoptosis, the mechanism may be related to reduced expression of Survivin mRNA closely related. Chinese medicine, blood stasis is one of the major causes of cancer, GFW is a prescription blood circulation, are widely used in the treatment of a variety of blood stasis syndrome, clinical practice, to prove a certain degree of anti-tumor effect, but the mechanism of its anti-tumor The study does not in-depth. This selection of tumor-bearing mice were used as object of study, observation GFW (GFW) on tumor cells induced apoptosis, from the molecular level, to explore its anti-tumor effect of GFW mechanism for the GFW and provide a theoretical basis for clinical application. 1 Materials and methods 1.1 The main instruments and reagents in situ hybridization kit purchased White Biotechnology Co., Ltd., Tianjin Hao Yang. OLYMPUS microscope (Japan), HITACHI-H500 type transmission electron mi