P16 Ki67 in cervical lesions and its significance.docVIP

 PAGE \* MERGEFORMAT 9 P16 Ki67 in cervical lesions and its significance Author: Wang Pei Fei Chen Jianwu Huang Ju-Dan Chen Ying [Keywords:] P16 Ki67 expression in cervical lesions Cervical cancer is a serious threat to women’s health, cancer, accounting for the top of women’s cancer, its incidence has been increasing and the trend of younger onset of cervical cancer, early diagnosis, early treatment, especially in the precancerous lesions found in cancer populations high-risk individuals to carry out highly targeted focus on prevention to reduce cervical cancer incidence and mortality, of great significance. This study used immunohistochemistry to detect P16 gene, Ki67 antigen in cervical intraepithelial neoplasia (cervical intraepithelial neoplasia, CIN) and cervical squamous cell carcinoma, and explore the relationship between the occurrence and development of cervical cancer. Report are as follows. 1 Data and methods 1.1 General data collection, Yueqing City People’s Hospital from June 2004 to October 2008 between the cervical biopsy, cervical Leep surgery, and hysterectomy specimens of 100 cases of patients aged 28 to 65 years old, mean age (42.12 ± 3.12) years of age. CIN Ⅰ ~ Ⅲ grade of which 70 patients, aged 28 to 60 years old, mean age (42.20 ± 3.61) years of age; 30 cases of squamous cell carcinoma, aged 30 to 65 years old, mean age (41.93 ± 3.08) years of age; 20 cases of normal cervical mucosa in patients uterine smooth muscle tumors from the surgical resection specimens, aged 32 to 70 years old, mean age (42.60 ± 3.50) years of age. The age difference between the three groups were not statistically significant (P all amp;quot;0.05). 1.2 means the entire organization by 10% neutral formalin-fixed, paraffin-embedded, 4um thick serial sectioning, immunohistochemical staining using EnVision two-step, DAB color to PBS buffer (0.01mol / L, pH7. 4) instead of first antibody as negative control, using a known positive tissue sections as positiv