Paliurus Aseptic Germination of seed_0.docVIP

 PAGE \* MERGEFORMAT 10 Paliurus Aseptic Germination of seed [Abstract] Objective To study the treatment of aseptic paliurus seed germination. Methods of concentrated sulfuric acid pretreatment of seeds with orthogonal design affect the germination of GA3 concentration, soaking temperature and soaking time to study, then will be disinfected explants were inoculated on MS medium with different hormone combinations in the sterile culture. paliurus seed germination results the best treatment is: concentrated sulfuric acid 9.5h +1000 mg / L of GA3 solution + pretreatment 24h + soaking temp