Paliurus Aseptic Germination of seed_0.doc

 PAGE \* MERGEFORMAT 10 Paliurus Aseptic Germination of seed [Abstract] Objective To study the treatment of aseptic paliurus seed germination. Methods of concentrated sulfuric acid pretreatment of seeds with orthogonal design affect the germination of GA3 concentration, soaking temperature and soaking time to study, then will be disinfected explants were inoculated on MS medium with different hormone combinations in the sterile culture. paliurus seed germination results the best treatment is: concentrated sulfuric acid 9.5h +1000 mg / L of GA3 solution + pretreatment 24h + soaking temperature of 35 ℃; the optimum seed germination hormone combination: MS +6- BA0.5mg / L + NAA0.1mg / L + GA3 30 mg / L; on paliurus seedlings were cultured in vitro, the liquid medium than in solid paliurus medium more conducive to in vitro culture. Conclusion paliurus seeds were further sterilized integrated approach to training, can improve paliurus seed germination rate. [Keywords:] paliurus seed; hormone; germination; sterile culture Paliurus Cyclocarya paliurus (Batal.) Iljinskaja also known as a cash cow, valley tree (Zhejiang), Maliuchang (Hubei), Li Ching and other money, the Department of Juglandaceae paliurus species, rare species endemic to China, is a key state protection of endangered species [1,2], in Guilin, Guangxi also have a distribution. paliurus have lower blood sugar, blood pressure, blood fat and so on, are considered safe treatment of type 2 diabetes and low toxicity of the medicine. Existing paliurus more scattered in the deep forests and a number of nature reserves, mainly natural, paliurus poor seed development, seed abortion is very serious and has a deep dormancy, low germination rate of artificial breeding, the general planting to be next year or even after 2 years after germination [3], hard husk that have hindered one of the factors seed germination [4], which to some extent, restricted the paliurus Rengong the cultivation and utilization o