Participation oral solution in the Determination of ginsenoside Rg1.doc

 PAGE \* MERGEFORMAT 11 Participation oral solution in the Determination of ginsenoside Rg1 【Abstract】 Objective high-performance liquid chromatography (HPLC) Determination of reference oral solution in the content of ginsenoside Rg1. Method of column packing for the 18 alkyl silane bonded silica, mobile phase of acetonitrile  0.05% phosphoric acid. The results of ginsenoside Rg1 between in the 0.251 ~ 2.15 μg showed a good linear relationship; preparation of ginsenoside-Rg1, the average recovery was 98.73% (RSD = 2.7%). Conclusion The method is simple, accurate and good separation effect, non-interference, can be used to reference oral solution quality evaluation. Keywords: ginseng oral solution; ginsenoside Rg1; high performance liquid chromatography Abstract: ObjectiveTo establish the quantitative method of ginsenoside Rg1 in Shenhuang Oral Liquid by HPLC. MethodsThe column was packed with 5 μm Diamonsil C18 stationary phase. The mobile phase consisted of acetonitrile-water.ResultsThe linear range was 0.251 ~ 2.15 μg.The average recovery was 98.73% (RSD was 2.7%). ConclusionThe method was convenient and accurate. It can te used as a method of quality evaluation for Shenhuang Oral Liquid. Keywords:: Shenhuang Oral; Ginsenoside Rg1; HPLC Participation oral solution is based on ginseng, Astragalus, Bupleurum, etc. 8 flavor compound preparation of Chinese medicine made of herbs, the Department of Traditional Chinese Medicine six categories of new drugs, with a spleen Qi, Jieyu refreshing effect. Fang ginseng-based drug, in this experiment, high-performance liquid chromatography reference oral solution in the determination of ginsenoside Rg1 to conduct research. An instrument and reagent Instruments: high-performance liquid chromatography: Shimadzu (LC-2010A), acetonitrile HPLC pure, Dikma company. Reference substance ginsenoside Rg1 (lot number 0703-200118), China’s pharmaceutical and biological product