Pervanadate on irradiated BET.doc

 PAGE \* MERGEFORMAT 4 Pervanadate on irradiated BET [Keywords:] BET-2 cells; ionizing radiation; mitochondrial transmembrane potential (ΔΨ m); apoptosis; pervanadate Abstract: Objective To study the tyrosine phosphatase inhibitor pervanadate (Pervanadate, Per) After the effects of ionizing radiation on mitochondrial membrane potential (ΔΨ m) changes and cell apoptosis. Methods The effects of ionizing radiation detected by flow cytometry after the murine myeloid leukemia cell line BET-2 cells, mitochondrial membrane potential and its application in pervanadate change after the intervention and cell apoptosis. Early results of ionizing radiation can cause BET-2 cells reduced mitochondrial membrane potential and induce cell apoptosis; pervanadate a certain extent, stability of mitochondrial membrane potential and reduce apoptosis. Conclusion pervanadate reversible ionizing radiation-induced cell reduction in mitochondrial membrane potential, reducing the occurrence of apoptosis in hematopoietic cells. Keywords: BET-2 cells; ionizing radiation; mitochondrial transmembrane potential (ΔΨ m); apoptosis; pervanadate Effects of pervanadate on MMP of BET-2 cells induced by ionizing irradiation Abstract: Objective To study the effects of pervanadate (Per), the inhibitor of protein tyrosine phosphotases on the alterations of mitochondrial membrane potential (MMP) and the apoptosis of BET-2 cells induced by ionizing irradiation.Methods BET-2 cells were divided into Per-treated groups and non-Per groups and incubated after ionizing irradiation.The MMP and apoptosis were measured with FCM.Results After irradiation was given, the MMP of BET-2 cells was reduced in the early stage significantly, and the apoptosis appeared, and these changes had a dose-dependent pattern and a time-course fashion.Per potently enhanced the MMP of BET-2 cells and prevented irradiation-treated cells from apoptosis.Conclusion Per reversed the repression of mitochondrial membrane potential and su